FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6203136
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG22000.020.7602311
CTATACT19350.020.6072714
CGTCTTA16200.019.692348145
GTACTAG11950.019.4131661
GTCCTAA19100.018.9780221
GTGTTAG23950.018.7672421
GTACTAA16850.018.5004731
CTAAGCG7650.018.0065694
GTATAGG17100.017.8060461
GTACTAT18500.017.2423021
TCTAGAC21950.017.1751543
GTATTAG20350.017.0998041
GTGCTAG22050.017.0965731
CGCTATA5652.7703209E-916.6811772
GTACAGG43850.016.5327361
GTATATA32200.016.2102951
GTGTAGA34700.016.0870171
GTACATA24550.015.9461771
TAGTACT18250.015.8904394
GCTCTAA28750.015.8860881
Produced by FastQC (version 0.11.5)