FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6203136
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA12400.028.637348145
CGCCGTT14150.025.095629145
CCGACGC95850.023.892048145
GTCCTAG23650.023.6515181
GTACTAG12000.021.1878191
TTACGTT15950.020.900448145
GTACTAA15350.020.823021
GTGCTAG22900.019.9850161
GTTCTAA27700.019.668941
GCCGACC27750.019.32534145
GTGCTAA23000.019.266441
GTATAGG16250.019.2227081
GTATTAG19750.019.1265091
GTCTTAG20900.019.1168271
GACGACA30350.019.102478145
GCCCTAG20850.018.4658471
GTTCTAG27800.018.2916411
TTATACC56550.017.813145145
GTCCTAT22000.017.5005871
GTACATA25750.017.4909691
Produced by FastQC (version 0.11.5)