FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6100309
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG22250.023.1375981
GTATTAA28750.021.437351
GTGCTAG20200.020.1014141
GTACTAG11600.019.3773171
GTACTAA18150.017.1783641
ATAGTAC19150.017.0381723
GTATAGG16750.016.8826181
GCCCTAG24250.016.744271
GTCTTAG20000.016.6769941
GTATTAG20100.016.5940251
TGCCGTG35950.016.536667145
GTTCTAG25700.016.3638251
CGTCTTA24600.016.209164145
TCTATAC22000.016.1492613
GTATAGA19850.016.0724531
CGTCTTG29450.016.001514145
GTGTATG34950.015.97473051
GCTCTAG26350.015.9601621
GTCTTAA24100.015.9458911
CTATACG4551.3732151E-615.9355814
Produced by FastQC (version 0.11.5)