FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6100309
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA16500.024.60466145
CGCCGTT15550.022.378145145
GTCCTAG21600.022.1711461
TTACGTT20750.021.661417145
GTACTAG10400.021.6284941
GTTCTAG26250.020.4550421
GTATTAG18600.019.5054071
GCTCTAG28550.019.0613271
GTGCTAG22050.018.4279651
GTTCTAA25600.018.4234681
GTACTAT18800.018.1400281
GTTCTAT26250.017.4144271
CTAGACA25400.017.4102844
GCCGACC27900.017.409403145
GCCCTAG23400.017.0547281
GTACAGG42350.016.9621051
GTACTAA15850.016.9383241
TCTAGAC19800.016.8434413
GTACATA24750.016.417641
GTCCTAA20150.016.2044931
Produced by FastQC (version 0.11.5)