FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6061651
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18800.021.9828451
GTCCTAG21300.021.4450871
GTGCTAG20950.020.7651021
GTTCTAG25800.018.8287721
GCTCTAG28550.018.5388851
GTCCTAA19600.018.1262041
GTACTAG11000.017.7966381
GTACATA26650.017.1399751
TGCCGTA26850.017.012444145
GTATATA29950.016.9460351
CTATACT21050.016.533714
TATACTG28100.016.5140975
TCTAGAC19850.016.4374033
GTCTAGA19500.016.3600621
GTGTATA24650.016.1775461
GTATAAG22000.016.1488021
TGCCGTG42250.016.131382145
GTATAGA20850.015.9962671
GTACTAA17450.015.7890151
GTACAGG43500.015.6677071
Produced by FastQC (version 0.11.5)