FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8331458
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT14400.025.167597145
TATACCG5150.023.9316355
GTACTAG15650.023.6805361
GTATTAG28000.023.0976411
GTCCTAG32450.020.8259071
GTTCTAG41350.020.3853471
CCGTATA13750.019.504429145
GTACATA34150.019.1508241
GTTCTAA40400.019.0660251
TTATACC66550.018.40657145
GTACAGG61250.018.0332161
GTCCTAT29850.018.0145341
CTGCCGC142750.017.822361145
GTATACG6553.6379788E-1217.7506541
GTGCTAG34250.017.6097491
GTCCTAA27800.017.2518161
GTATTAA45650.017.1916921
GTATTAT44450.016.8384131
GTACTAA26950.016.7173941
GTATATG42000.016.6095391
Produced by FastQC (version 0.11.5)