FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8251805
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27700.021.9842341
GTCCTAG32650.019.3173661
CGTCTTG40600.019.285603145
CGTCTTA23200.019.062391145
GTATAGG23400.018.5886231
GTCTTAG30550.018.5095231
GTATTAA43750.018.3931771
GTTCTAG38550.018.2414441
TGCCGTG48900.018.236092145
GTACTAA25950.018.1588291
GTGCTAA34200.017.593971
GTACATA35550.017.537621
GTACAGG58450.016.8681011
CACGAGC123000.016.798685145
CTACATG104100.016.784246145
GGTCTAG25600.016.7079771
GTACTAG15450.016.4229581
GTGCTAG30550.016.373811
GTATAAA55850.016.2255231
GGCTTAG33800.016.0863081
Produced by FastQC (version 0.11.5)