FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8116134
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG23150.020.9834731
GTCCTAG34400.020.8655741
GTATTAG27800.019.2992521
CGTCTTG50100.019.102482145
TACGCGA3151.6851454E-518.4136982
GTTCTAG37100.018.369931
GTATTAA40000.018.125651
GTACTAC22350.017.5174061
GTACTAA25400.017.1265981
CTAGACT28900.017.0597524
GTGCTAG32050.016.9662861
GTACAGG62050.016.9425891
GTATATA48550.016.8749621
CGAATCG5652.763045E-916.6839689
TGCCGTG58050.016.486378145
TCTAGGC39200.016.2763943
GCTCTAG39800.016.2128931
CTATACT30650.016.0857034
GTATAGA31850.015.9346361
GTACATA34950.015.7659441
Produced by FastQC (version 0.11.5)