FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8116134
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29200.022.8616161
GTCCTAG33200.022.0742151
CCGTATA19350.020.979578145
GTACTAA26350.019.5514811
CGCCGTT19550.019.281742145
GTGCTAG32400.019.0360091
GTTCTAA38900.017.9070321
GTTCTAG38550.017.8813861
GTATTAA45600.017.1854481
GTACTAG16650.016.996221
CTCCTAG48450.016.6238331
GTATAGG24500.016.5853231
GTATATA51550.016.4686931
GTCTATA28200.016.4676951
GTCTTAG31800.016.2006781
TCTAGGC42300.016.1126943
GCCGACC34200.015.897354145
GCTCTAG38350.015.8933661
GTCTAGG31050.015.8909281
GTCTTAA39950.015.8017231
Produced by FastQC (version 0.11.5)