FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10481213
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12550.015.4210521
GTCCTAG24750.014.5220461
GTATAGG16550.013.9909271
GTTCTAA27750.013.6993321
GTATTAG17450.013.6654321
GTACTAA19550.013.6117391
GTGCTAG22650.013.5797411
GTTCTAG28300.013.5552091
CCTATAC15900.013.4761023
GCTCTAG29350.013.305771
GTATTAA30400.013.3009291
GTCTTAG22000.012.8813351
GTCTTAA26100.012.7116061
GTATAAA40500.012.5438821
GTATATA29000.012.5129861
GTACATA26150.012.4229821
GTGTAGG36050.012.3667091
GCCTTAG23550.012.327021
GGTCTAG21400.012.1120371
GTCCTAA20850.012.1000311
Produced by FastQC (version 0.11.5)