FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10481213
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG22200.019.3469471
GTACTAG12550.018.215621
GTCTTAG23400.017.0225941
GTACTAA19650.014.9830461
GCTCTAG29700.014.8112261
GTGCTAA24750.014.6946021
GGTCTAA18650.014.672091
GTATTAG19050.014.3640151
CTATACT23550.014.2400394
GTATTAA31050.013.83261
TATACTG28650.013.7519245
GTGTTAG24250.013.7120761
GCTCTAA28800.013.3497841
GTTCTAG29050.013.2348981
GTCCTAA21250.013.0399161
GTCCTAT22350.013.0180371
GTACATA29350.012.9816031
GTACAGG47700.012.9254411
GTGCTAG22550.012.9025771
GTATATA31000.012.8493121
Produced by FastQC (version 0.11.5)