FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10846861
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG3251.2187229E-1015.9472871
GTACTAA18450.015.9184741
GTCCTAG26400.014.7894961
GTATTAG17550.014.7660051
GTCTTAG23150.014.3284461
GTATAGG17200.014.2629311
GTACTAG12050.014.0503741
GGTCTAG20300.013.9571481
GTATTAA32750.013.7154781
GTATATG27950.013.10397151
GTTCTAG28550.012.9496071
GTATATA33900.012.9444281
CCCCTAG26450.012.8020431
GTGCTAG23350.012.7259561
GGTCTAA18850.012.6478471
GGTTATA21050.012.6391391
GTACTAT20850.012.5946591
CCCTTAG30200.012.5853311
GCTCTAG31350.012.3440981
GTCTAGA24100.012.3299211
Produced by FastQC (version 0.11.5)