FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10846861
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12000.018.154821
GTTCTAA30800.016.2798331
GTCTTAG24150.015.7510021
GTCCTAG26200.015.1785121
GTGCTAG26250.015.0178641
GTATTAG21200.015.006681
GTACTAA19700.014.92056751
GGTCTAG20350.014.7838481
TATACTG29250.014.1749495
GTCCTAA22750.013.8322441
GTATTAA30650.013.7645491
GTTCTAG29550.013.3407421
TACGCGA2851.5826099E-613.3390252
GTATAGG17150.013.3079891
GTATACG5200.013.3002341
GTGTTAG24100.013.0574081
GCTCTAG32000.012.9677281
GTCTATA18350.012.8146131
GTATATA33750.012.7051731
CCTCTAG42300.012.6713821
Produced by FastQC (version 0.11.5)