FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10716461
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12300.017.1371481
GTATTAG18850.016.1318861
GTACTAA20050.015.6834221
GTGCTAG25250.015.19061851
GTATTAA31100.013.9998691
GTATAGG16700.013.8634781
TACGCGA2001.9568355E-413.8223512
GTCCTAG26550.013.7960631
GTACTAT18600.013.5620011
GTATAGA22650.012.9677481
GTACATA26700.012.9420351
GGTCTAG18200.012.9107471
GTATAAG20700.012.853881
GTTCTAA33100.012.8407671
GCGCTAA3508.748793E-812.83481
GTATATG29350.012.8331181
CTAGTAC10900.012.6810573
GTTCTAG28150.012.5209031
GTATTAT30300.012.3167171
GTCTTAG23300.012.3093761
Produced by FastQC (version 0.11.5)