FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10716461
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG27550.017.3188021
GTACTAA20050.015.3474431
GTATTAG18500.014.5775061
GGTCTAA18900.014.2689891
GTATTAA33050.013.9136331
GGTCTAG19050.013.7936421
GTACTAG12650.013.6659511
GTTCTAG31450.013.5221211
GTGCTAG22850.013.4667941
GTCTAGG25250.013.4191511
GTATAAG21250.013.3418261
CTAGACT20600.013.2524194
GTCTTAG24100.013.1987011
GTATATG32250.013.079481
TCAGTAC30500.013.0315533
TCTATAC20750.012.991963
GCTCTAG32500.012.978871
GTGCTAA27450.012.8474871
GTATTAC20450.012.6802651
GCTCTAA30750.012.48067951
Produced by FastQC (version 0.11.5)