FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9593751
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT19750.045.151676145
CGCCGTT19450.029.074406145
CCGTATA20800.025.793142145
GTACTAG19200.024.9463311
GTCCTAG40350.024.819871
GACGATA43900.021.13885145
GTATTAA53150.020.2079561
GTCTTAG38400.019.276711
GTGCTAG39550.019.2666741
GTTCTAG44200.018.7174431
GTATTAG34700.018.6133481
GTCCTAA34700.018.404211
GGTCTAG30550.018.2912541
GCCGACC39900.017.988632145
CTAGACT34050.017.6725464
GTCTTAA45300.017.4619311
TCTATAC37350.017.2768553
CGACGAA30900.017.127747145
CTATACT37700.017.1153894
GTTCTAT41850.016.4737361
Produced by FastQC (version 0.11.5)