FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6404958
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG16550.017.443381
GTATTAG13200.016.6630861
GTGCTAG14100.015.3557421
GTACTAA12200.014.9301921
GGTCTAA11300.014.902771
GGTCTAG12700.014.8836121
GTCCTAG14550.014.8808211
GTATAGG9800.014.7289781
GTACATA18500.014.67589951
GTACTAG7350.014.4951821
GTACAGG26150.014.0624661
GTCTATA11300.013.6862171
GTACATG23150.013.5095161
GTGCTAA13500.013.4924711
GTATTAA19200.013.4248491
TCTAGAC13450.013.2876253
GCCCTAG15300.013.252871
CTAGACT12300.013.1332634
CTATACT14400.012.8887614
TATACTG17700.012.8160425
Produced by FastQC (version 0.11.5)