FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6404958
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA13100.018.4180011
GTACTAG7000.016.7415871
GTATTAG11650.016.5682951
GTATTAA19250.016.1148951
GTCCTAG14450.015.98168851
GTACATA16950.015.2513151
GTTCTAG17000.015.0037051
GCTCTAG19100.014.9782281
GTGCTAG14500.014.73802951
GTCCTAA13350.014.7166611
GTGCTAA15750.014.6625661
CTAGACT12800.014.5080054
GCTAGAC10000.014.1101043
TTATACT21200.013.9503954
GTCTTAG14300.013.738981
CCTATAC11950.013.5355623
GTATATA21850.013.2508461
CTATACG3651.1334123E-813.19043354
CTTATAC14950.013.1213953
TATACTG17850.013.0964055
Produced by FastQC (version 0.11.5)