FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6525197
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG15650.016.0374661
GTCCTAG15150.015.8859281
GTATTAG12350.015.3116841
GTATTAA19000.015.0193691
GTATATA20350.014.5298531
GTTCTAG17100.014.4765011
GCTCTAG17400.014.2269061
GTACTAA11450.014.1130081
GTCCTAT14450.014.0381981
GTCCTAA14450.014.0381981
GCCTTAG15200.014.024111
GTCTTAA17600.013.6745361
GTATAGG10650.013.5589761
TATACTG17950.013.2171715
GTCTTAG14050.013.21431351
GGTCTAA10200.013.145941
CTAAGGT14250.013.029454
GCCCTAG15250.012.8508611
GTATTAC14750.012.8202911
GTCTAGG13700.012.7990231
Produced by FastQC (version 0.11.5)