FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6464141
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7750.018.2011261
GTGCTAG15350.017.930721
GTACTAA11750.017.5682891
GTCCTAA12650.016.8623181
GTCCTAG15250.016.6946751
GTTCTAG18100.015.7766791
GTATTAG12850.015.261171
GTATTAA18450.015.10444451
GGTCTAG11150.014.8109351
TAGTACC8400.014.7315844
GTCTTAG14750.014.6948321
GTGCTAA15800.014.1537781
CCTCTAG25400.013.9514581
GCTCTAG18250.013.7618271
GTTCTAA18700.013.6146421
GGTCTAA12550.013.4328591
GTATATG16700.013.3910011
GTATAAG12500.013.2113541
TATACTG17750.012.9747595
CTAGACT12150.012.7309994
Produced by FastQC (version 0.11.5)