FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6443474
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG14750.015.6248331
GCCCTAG14900.015.4675361
GTGCTAG16550.014.7568261
GTCCTAG14500.014.708091
CTAGACT13100.014.695344
GTACTAA12350.014.4833491
GTTCTAG17900.014.4125511
GGTCTAA11250.014.37071
TCTAGAC14200.014.2840093
GTTCTAA19250.014.1165621
TATACTG18900.014.0050635
GTACTAG7250.013.7591811
GTATAGG10350.013.6262431
GTATTAA18500.013.5732461
GTCTTAA18600.013.1304021
GTATAAG12700.013.0008011
TAGACCG3204.3751606E-712.8910245
GCTCTAA18300.012.7817531
GTCCTAC12700.012.7299511
GCTCTAG18400.012.7122871
Produced by FastQC (version 0.11.5)