FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4795059
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA29100.027.891537145
CCGTATA6255.631935E-627.546698145
CCTGTCG13002.2711538E-718.919436145
GTCCTAG15400.018.158911
GAGCGAT10002.1134908E-417.216684145
TCTAGAC16450.016.3023913
GTATTAA23750.016.099991
GTACTAA13450.015.2754741
CTAGACG3852.9213115E-614.8203544
GTCTTAG17750.014.468671
CTAGACT13650.014.2123414
GTATAGG14550.014.1206271
GTACAGG28650.013.94401551
GTACTAG8600.013.9359081
GTATTAG18500.013.8821031
GTCTTAC21400.013.6010011
CGACGAA10905.654359E-513.538677145
TAGACAG26200.013.5023625
GGTCTAG15300.013.4284381
GGTCTAA15100.013.2283461
Produced by FastQC (version 0.11.5)