FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4835302
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA30650.044.561283145
GTACTAA14850.019.189351
GTCCTAG17950.018.7328681
GTATTAG15350.016.7078591
GTTCTAG18750.016.4138031
GGGGGGG263350.015.4661875145
TATACCG4858.87303E-915.2769375
GTCCTAA17000.015.0862161
GTACAGA34300.014.6219471
GTACATA20000.014.5330531
GTGCTAG18700.014.3242851
GGTCTAG13150.014.3022681
GTGTTAG18800.014.2480931
GTATAAG17400.014.0843211
CTATACT18500.013.8634384
GTATTAA25250.013.542741
GTCTTAA21600.013.4565311
GTTCTAA24650.013.1787621
GTCTATA15250.013.0802151
GTACAAG28850.013.03811551
Produced by FastQC (version 0.11.5)