FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4848750
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA33250.055.405098145
GGGGGGG205550.019.8116145
GTCCTAA15500.018.4064351
GTACTAG9350.017.697741
GTATTAG15900.017.2256471
GTTCTAG20200.015.81861
GTACTAA14700.015.5265181
GTCCTAG18750.014.9116681
GTGCTAG16550.014.8252441
GCTCTAG20650.014.3686081
CGGGGGG30500.014.305414145
GTCTAGA16400.014.2649891
GTCTTAA20950.014.1628511
GTCTTAG17650.013.254721
GTACATA20300.012.9298421
GTACAGG30200.012.8479371
GTGCTAA18950.012.6465331
CTAGGAC15100.012.4700563
TAGTACA18350.012.4381364
TCTAACC16150.012.3659343
Produced by FastQC (version 0.11.5)