FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9335558
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA65450.022.873604145
GTTCTAG41050.017.498381
GTACTAG18050.017.0552081
GTCCTAG36700.016.9408511
GTGCTAG37800.015.6494241
GTCTTAG32850.015.4350471
GTACTAA28600.015.4070991
CTATACG6303.6379788E-1115.3064764
CTATACT33450.015.1348784
GTATTAG30000.014.8893081
GTATAGG22950.014.7288691
GCTCTAG43850.014.4538571
GTCCTAA32650.014.2354631
GTATTAA45100.013.7855691
TCTATAC34650.013.5711753
GTCCTAC33450.013.1731851
TATACTG44500.013.135095
GTACTAT26850.013.0391041
CTAGACT31500.013.0105054
CTGTACT69850.012.8562894
Produced by FastQC (version 0.11.5)