FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9058123
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA65700.030.354822145
GACGTAT11305.7843863E-1022.06097145
GTACTAA27900.017.4707981
GTACTAG17600.017.437711
GGGGGGG531300.017.360611145
GTCTTAG32150.017.0330491
GTATTAG29900.016.5034471
GTCCTAG34600.015.1312621
GTTCTAG43600.015.0442991
CGCGTAT5606.0312427E-414.838629145
GTATATG42650.014.6739241
GTATTAA45200.013.9792171
TATGCCG23900.013.907335145
GTGCTAG33950.013.6484381
GCCCTAG36400.013.2257591
CTAACCT37850.013.0372124
GGTCTAA28700.012.9999531
GTGCAGG129450.012.9698261
TATACTG40000.012.9385265
TGCACTG105000.012.8382275
Produced by FastQC (version 0.11.5)