FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9058123
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA66350.028.537191145
GTACTAG16550.018.9374141
TACGCGA2702.2050346E-517.8472082
GTTCTAG43700.017.3781871
GTATAGG25200.015.7855151
GTCCTAG38700.015.5741511
GTCCTAA32400.014.8819671
CCTGTCG29700.014.875529145
GTATTAG31300.014.8272891
TATACTG43600.014.7690595
CTATACT34400.014.3477194
GTCTTAG32800.014.3329681
GTACAGG68950.014.2484841
GTATTAA50100.014.0754881
GCTCTAG43100.013.8443721
GTACGCG6156.522896E-913.7204481
GTGTTAG36700.013.6309891
CGACGAA50350.013.370883145
CGCCGTA17350.013.33837145
TGTACCG13100.013.3223925
Produced by FastQC (version 0.11.5)