FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8923390
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA71950.033.345936145
GGGGGGG491450.018.266674145
GTATTAG31550.017.8145221
GTACTAG18450.017.6883771
GTGCTAG32250.016.1160771
GTTCTAA42650.015.303661
GTCCTAG36950.014.8839261
GACGTAT13703.426203E-614.593918145
GTACTAA27400.014.5574051
GGTCTAG28700.014.5297391
CCTGTCG34850.014.342661145
GGTCTAA28350.014.2827661
GTACATA39000.014.1015681
GCCCTAG36950.014.0661291
GTTCTAG41750.014.0412531
TCGTATG31900.013.788735145
CTATACT31750.013.515424
GGTTACG8151.0913936E-1113.34767151
CGAATCG7756.730261E-1113.2573689
GTACAGG70800.013.2308881
Produced by FastQC (version 0.11.5)