FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8923390
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA67050.028.593374145
GTCCTAG36150.018.3625431
CCTGTCG30800.017.879253145
GTACTAT26950.015.4503651
GTTCTAA44950.015.3046571
GTCTTAG33550.015.2888511
GTATTAG31200.015.2799161
GTCCTAT33350.015.1995911
GTGCTAG33250.015.0638131
GTACTAA28050.015.0596071
GTACTAG18100.015.0031481
GGTCTAA27400.014.3156561
CGACGAA65200.014.076712145
CTAGACT30500.014.0398844
GTATTAA45150.013.9002961
GTACATA40450.013.7251711
GCTCTAG44450.013.3046291
GTATATA53200.013.2715821
CTAGGCC41850.013.2585914
CTGTACT67600.013.2044414
Produced by FastQC (version 0.11.5)