FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8377835
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA68350.034.832397145
GTACTAG15650.019.2171971
TCGCGTT5350.005710836519.181393145
GTCCTAG32250.016.5994851
GTATTAA43500.016.3165041
GTGCTAG35950.015.8949471
CCTGTCG29700.015.203031145
TGACGCG10909.4139046E-515.063553145
GTCCTAA29600.015.0374561
GTATTAG28500.014.7736411
GTACTAA25850.014.6593371
GTTCTAG39250.014.4053081
GTCTTAG32200.014.3836541
GTTCTAA41550.014.3317271
CGACGAA68150.013.853385145
CCGTATA8950.00666104213.759167145
CTATACT31700.013.6556734
TATACTG39850.013.5809225
CCGACGA70600.013.372638145
GGTCTAA27700.013.245991
Produced by FastQC (version 0.11.5)