FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506989
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21100.016.659081
GTCCTAG27750.016.2682781
GTGCTAG26200.015.3892651
GTTCTAG29650.014.6446561
GTACTAG12950.014.6361281
GTCTTAG24500.014.4878661
GTACTAA19800.014.4459531
GTATTAA34900.014.3177871
GTTCTAA32250.014.10514451
GTATATA37000.014.0639891
GTATATG33200.014.0129331
CTAGTAC13400.013.3729173
TATACTG33550.013.3531635
GTACTAT21200.013.1668671
GGTCTAG21700.013.0222921
GTACATA30050.012.6148351
GTGCTAA24600.012.6078411
GGTCTAA22500.012.5592771
GTCTATA19100.012.4494171
ATAGTAC19900.012.2951243
Produced by FastQC (version 0.11.5)