FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506989
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA22150.017.1525521
GTACTAG13150.016.284551
GTCTTAG26850.015.8223361
GTATTAG21500.014.9401261
GTTCTAG31950.014.377741
GTCCTAG29000.014.0538011
GTCCTAA24700.013.8435681
GTTCTAA30600.013.7704531
GCCCTAG26900.013.6101661
GTGCTAG25750.013.2790741
GTACAGG52500.013.2235031
TCTGTAC48600.013.0549483
GTATAGA22100.012.9716621
GTGCTAA27750.012.9443471
GTCTTAA31000.012.9242721
GGTCTAA22050.012.8444371
GTACAAG43100.012.8219031
GCTCTAG32800.012.636221
GTCCTAT24900.012.6226871
GTATAGT19200.012.5923451
Produced by FastQC (version 0.11.5)