FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11491572
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13500.018.1270711
GTATTAG22400.016.156391
GTACTAA20650.016.0233821
GTCCTAG28150.015.6723671
GTGCTAG28400.015.5344071
GTTCTAG32800.015.4470851
GTATAGG17550.015.3186511
GGTCTAA21900.014.7940391
GTATTAA34350.014.64971351
GTTCTAA30900.014.5006651
CTATACT26650.014.4847364
GTCTTAG25250.014.1962941
TATACTG34650.013.9256235
GTACTAT20200.013.8209111
TCTATAC23300.013.6088583
GTATATA39550.013.595061
TACGCGA2304.316971E-513.4866622
GTCCTAA24850.013.1765061
GTGTTAG28100.012.8791141
GTACAGA54500.012.8381551
Produced by FastQC (version 0.11.5)