FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10882663
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG3650.017.9385031
GTATTAG20800.017.5617581
GTCCTAG25450.016.5195241
GTTCTAG29650.015.2255171
GTCTTAG23400.015.1686471
TCTATAC21450.014.619673
GTATATA35000.014.4735391
GTATAGG17700.014.4073441
GTACTAG12450.014.3932691
GTACTAA20950.013.4882391
GTACAGG49100.013.4755111
GTATTAA31000.013.4508311
CTATACT25650.013.3006624
GTGCTAG25450.012.9989691
GTATAAG22550.012.9896621
GGTCTAA19150.012.7765921
TATACTG32550.012.3869485
GTTCTAA29500.012.2657111
GCTCTAG30950.012.2477831
GTCCTAA22050.012.1902171
Produced by FastQC (version 0.11.5)