FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10882663
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG25700.015.9672111
GTATTAG20650.015.5302371
GTCCTAA22650.015.3768851
GTATATA32850.014.801271
GGTCTAA21500.014.7558621
GTACTAA19950.014.3466461
GTGCTAG26700.014.077661
GTATTAT28700.014.0578591
GTCTTAG25100.014.0133411
GTTCTAG30850.013.8605921
GTACTAG12250.013.5120221
GCCCTAG27450.013.4417751
GCTCTAG30950.013.25871851
GCTCTAA30500.013.2282141
GGTCTAG20950.013.1680411
GTCCTAT20850.013.0658071
GTCTTAA30100.013.0603131
GTCTATA21300.012.789771
GTATTAA28850.012.7894871
GTACAAG37150.012.7167741
Produced by FastQC (version 0.11.5)