FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18304486
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21250.017.85721
GTACTAA33000.016.8302861
GTCCTAG44400.016.6268651
GTATAGG28750.015.7185711
GTGCTAG40700.015.0870791
GTCCTAA36850.014.13575651
TAGTGCG7950.014.09933357
GTATTAA47100.013.9891751
GTATATA56000.013.7987451
GTTCTAG49650.013.7570561
GGTCTAG33250.013.4874941
GTATTAG34000.013.3928991
GTACAGG81400.013.3071431
GTATAAA64450.013.1136251
CTATACT39550.012.996434
GGTCTAA32750.012.9560741
TCTAGAC41700.012.90523053
TATACTG49650.012.8539555
GCTCTAG52750.012.7523951
GTTCTAA50400.012.6625381
Produced by FastQC (version 0.11.5)