FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18165020
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29850.017.1027471
GTCCTAG43650.015.7259651
GTCCTAA37900.014.5622761
GTACTAG18050.014.5239541
GTACTAA32550.013.9885091
GTATTAA45650.013.8279821
GTCTTAG38350.013.6718321
TAGTGCG9500.013.64056557
GTATATA57450.013.5695941
GTTCTAA47750.013.3643191
CGATCGT7500.013.34117438
GCTCTAG51350.013.3006621
GTGCTAG42300.013.2921651
CTATACT37600.013.1189154
GTTCTAG48200.012.8817231
GTACAGG84300.012.7256891
GTATAGG27150.012.7051171
TCTAGAC37600.012.6601753
GGTCTAG33300.012.6375851
GGTCTAA32300.012.4948721
Produced by FastQC (version 0.11.5)