FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18165020
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19950.016.807581
GGTCTAA31300.015.1304761
GTCCTAG46200.014.9645741
GTATTAG32700.014.9055391
GTTCTAG44650.014.8646991
GTATAGG26600.014.8149291
GTTCTAA50450.014.5261681
GTGCTAG44400.014.4812591
GCTCTAG57750.014.3061331
GTCCTAA39600.014.2288161
GTACTAA33550.014.1157641
GTCTTAG41150.014.1128841
GTGCTAA39500.013.8272671
TAGTGCG10350.013.53475657
GTATTAA45600.013.2662931
TCTATAC35600.012.990043
GGTCTAG34050.012.2841351
GTACTAT32100.012.1688561
GTATATA51150.012.1647511
CTATACT40950.012.1318764
Produced by FastQC (version 0.11.5)