FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18049353
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21200.017.423521
GTCCTAG44900.016.8378141
GTATTAG34600.015.3651
GCTCTAG55000.015.2521651
GTCTTAG38050.014.8791141
GTACTAA32400.014.7035431
GTATTAA48000.014.6715791
GTGCTAG42300.014.6083311
GTTCTAG47650.014.1997631
GGTCTAG34600.013.7686361
GTCCTAA39100.013.15521
GTTCTAA50650.013.0860841
GTATATA53350.013.0708821
CGAATCG9250.012.676779
GGTCTAA32300.012.6115191
GTACATA46200.012.5532231
CTATACT37250.012.4994854
GTGCTAA40350.012.1487821
GTCTTAA43900.012.111
GTACTAT32000.011.9745971
Produced by FastQC (version 0.11.5)