FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17837554
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19650.016.8654041
GTATTAG32900.016.788511
GTCCTAG43650.016.2127861
CGAATCG9150.016.2085159
GTACTAA32500.015.93294051
GTATTAA47200.015.4322231
CGATCGT8300.015.37825938
GGTCTAA34050.015.1062691
GTGCTAG41050.014.800881
GTCTTAG37100.014.7018241
GTCCTAA37600.014.5063221
GTGCTAA41300.013.7918331
GCTCTAG54000.013.744621
GTTCTAA48900.013.6249991
TCTAGAC37500.013.6149993
GTATAGG28950.013.5939081
CTAAGCG13200.013.589724
GTTCTAG51000.013.5377921
GCTCTAA50000.013.4633351
GCCCTAG46150.013.3148521
Produced by FastQC (version 0.11.5)