FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4389717
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG5950.016.7568261
TCTATAC8550.015.682463
GTATTAG7850.014.8908691
GTACTAA7050.014.6299521
GTACGTA1901.3213989E-414.4759541
GTATATA12250.014.3134661
GGTCTAA7750.014.1957721
GTCTAGA10500.014.0795881
CAGTACT17350.014.0693674
GTCTTAA11650.013.8701991
GGTTATA10850.013.6254071
GTACCGT2801.3744193E-613.5068886
ACAGTAC13500.013.4976393
TCTAGAC9700.013.468763
GTACTAG5150.013.3516061
TAGTGCG1850.001488074413.31444757
GGCTAGG9600.013.2507741
GTACATG16900.013.2232271
CCTCTAG16900.013.2232271
GTCTAAG8850.013.2082841
Produced by FastQC (version 0.11.5)