FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4333274
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8500.017.3929481
GTCTTAG9950.015.8949251
CATACGC2959.891664E-915.1516553
GTATAAG8900.014.6797041
GTACTAG3757.2759576E-1114.6694021
GCGCAAT2152.3405371E-514.3922181
CTAGCGT1450.005337308614.2272834
GTACGAT1709.360187E-414.1577056
GTCTATA7850.014.0153521
CTAGTAC3455.151378E-913.9523593
GTATAGG7400.013.938411
TCTAACC9950.013.4765983
CTATTAG6650.013.4423561
CGATCGT2052.512564E-413.42023438
ATTAGAC8750.013.3600963
GTTCTAA12400.013.3089331
CTAGGAC9350.013.238224
GCTCTAG12800.013.1616341
CTAACCT11300.013.0836444
TTAGACT9000.012.9889824
Produced by FastQC (version 0.11.5)