FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4428718
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA8450.017.0841081
GTACTAG4900.016.8350681
GTATTAG7750.016.4096661
GTGCTAG9650.016.3843881
GTACTAT7000.014.7306841
GTATAGG7300.014.5961561
GTATTAA11200.014.4237951
GTCCTAG9750.014.1011691
GGTCTAA7950.013.8351081
GTACTAC5900.013.39909651
GTCTTAG9300.013.3051351
TCAGTAC12450.012.9756223
GTACGTA1900.00217014612.6632191
GTATAAA15850.012.5776191
GTCCTAA7950.012.5380661
CTAGTAC4953.45608E-1112.4987623
CGAATCG2204.5905798E-412.496089
GTATATA14050.012.4765221
CTCCTAG16300.012.4412531
CAGTACT16400.012.3653924
Produced by FastQC (version 0.11.5)