FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4428718
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50360.11371236551977344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG12800.018.0074331
GTATTAG7400.015.3415481
CTAGACG1657.487156E-414.5815514
GTCCTAA8850.013.6054131
TAAACCG2304.4302695E-513.4491395
CTAGTAC4355.2750693E-1113.4331773
GTACTAG5650.013.395571
GTTCTAA12350.013.3709191
GGTCTAG8500.013.3561731
GTACAGG17050.013.3170041
GTGCTAG10100.013.2840411
GTATTAA12200.013.253331
TTGCGCG2355.4186126E-513.1637312
GTCTTAG10800.013.0601181
TAGTGCG2203.768684E-412.79181257
GTCCTAG9250.012.6451561
CCTATAC7350.012.6268623
GTATATA12850.012.5829291
GTCTATA7450.012.4679331
TCTAGGC12700.012.45010953
Produced by FastQC (version 0.11.5)