FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8394435
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9700.017.310191
GTACTAA14050.016.627211
GTCCTAA17700.016.0855941
GTCCTAG19550.015.8704011
TAGTCGG6500.015.1615249
GTATTAG14200.014.9092371
TCTATAC17950.014.8447943
GTTCTAA22950.014.791631
GTGTTAG20700.014.6360381
GTATTAA21350.014.5323821
CTATACT18850.014.5233124
GTGCTAG21050.014.0458711
GTATAGG12450.013.7798421
TCCGAGC136300.013.737617573
TAGTGCG5350.013.64476757
GCCTTAG19650.013.5605121
GGTCTAA13000.013.4776331
CGATCGT3555.1468305E-813.36610638
GTATAAG15700.013.0197941
GTCCTAC18950.012.9056931
Produced by FastQC (version 0.11.5)