FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8753513
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11250.017.8460221
GTATTAG14750.017.8185231
GTCTTAG18000.017.6432251
GTTCTAA23450.016.1890621
GTGCTAG20350.015.7851781
GCGTTAA2804.111098E-815.6442391
GTCCTAG23000.014.9187081
GTATTAA23550.014.2602831
GTCCTAA17100.014.08896351
TCCGAGC138700.013.99987873
GGATTAG16950.013.9982861
CACGAGA32150.013.96410873
TCTATAC17300.013.9251323
GTATATA26500.013.63705351
GTTCTAG25250.013.4447521
GGTCTAA15850.013.3576471
GTACTAA14500.013.3425591
ACGAGAC23600.013.30073173
GTGCTAA19350.013.2053011
CCGAGCC120450.013.18170373
Produced by FastQC (version 0.11.5)