FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8753513
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT3450.022.21662338
GCGATCG3600.020.27672837
CGACGAA17100.018.1455573
GTATTAG15400.017.3254091
CCGACGA25200.016.94855573
GTACTAG10400.016.5175571
TATACCG2603.023015E-715.444425
GTCTTAG19150.015.2687171
GTACTAA16150.015.1629481
GTCCTAG21250.015.1357891
TGACGCT136000.014.27973373
GGTCTAA16850.013.88231
GTTCTAG23450.013.8716621
GGTCTAG17500.013.784381
TATACTG25100.013.6711935
GTTCTAA22900.013.0875911
CTATACT19550.013.0729344
GCCGACG33400.013.00604872
GCCCTAG20800.013.0031831
GTATAGA19150.012.978411
Produced by FastQC (version 0.11.5)