FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8586327
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9050.017.7498871
GTATTAG14750.016.5834271
GTTCTAG23550.016.2775821
GTCCTAG21400.014.8421551
GTACTAA14650.014.4537941
GTCTTAG17700.014.4383081
GTATATA25550.014.43184951
GTCTTAA20750.014.4273731
TCCGAGC136050.014.21878973
CGAGCCC91400.013.81704673
GTGCTAG23000.013.8096581
GCGATCG3808.651114E-913.44713337
GTCCTAT18300.013.3664241
GTATAGA18650.013.3113351
GCTCTAG24850.013.2223191
GTATAGG13600.013.1537231
GGTCTAA15900.013.0878781
CTAGGAC22350.013.0665513
GGTCTAG15100.012.5723921
GTGCAGG78800.012.5091861
Produced by FastQC (version 0.11.5)