FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8586327
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102740.11965535437911926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14600.017.0203381
GTACTAG10700.016.7349511
GGTCTAG16800.015.6615721
GCGATCG4200.015.64077737
CGACGAA17450.015.27113473
GTCTTAG19150.015.2662841
GTTCTAG25650.015.2443381
GTATAGG13350.015.0554441
GTATTAA22800.014.5854111
TGACGCT130450.014.495378573
GTGCTAA22050.014.418591
GTGCTAG22400.014.3564411
CGATCGT4402.3646862E-1114.10039738
GTACTAA15050.014.0832741
GTCCTAA18250.014.0167491
ACGCTGC90950.014.00769373
GTCCTAT19100.013.7756241
GTCTAGT12050.013.6470131
GCGCTAA3252.5120607E-713.4930461
TAGTGCG4604.9112714E-1113.48788557
Produced by FastQC (version 0.11.5)