FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698174
Sequences flagged as poor quality0
Sequence length79
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36200.016.741541
GCGAGAC54650.015.1639073
GTACTAG22500.014.7657271
GTCTTAG33950.014.40991
CGAGACT56350.014.3825034
GTATTAG40750.014.1555061
GTACAGG46200.012.9597851
GAGCGAG59300.012.9288691
AGCGAGA63400.012.7832372
GGTCTAG25800.012.4525671
GTTCTAG44650.012.4284811
CGAGACG16100.012.24215373
GTGCTAG38850.012.2165411
TCCGTAC6650.012.0774843
GTGCAGG77450.012.0674211
GTCCAGG87600.012.0028491
CCAGGAC88550.011.9559713
TCTAGAC35750.011.8456073
GTACAAG39050.011.7800041
GCCTTAG37550.011.6672161
Produced by FastQC (version 0.11.5)